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plasmid vector constructs 449 zebrafish liver zfl cells  (ATCC)


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    ATCC plasmid vector constructs 449 zebrafish liver zfl cells
    Plasmid Vector Constructs 449 Zebrafish Liver Zfl Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+construct/ZFL/pm42219039-231-0-9
    Average 95 stars, based on 179 article reviews
    plasmid vector constructs 449 zebrafish liver zfl cells - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Cyclic AMP (cAMP)-dependent proteolysis of GATA6 by proteasome: Zinc-finger domain of GATA6 has signals for nuclear export and proteolysis, both of which are responsive to cAMP.
    Article Snippet: .. Cell culture and transfection of expression plasmids Each plasmid construct was introduced into Cos-1 cells (ATCC, Manassas, VA, USA) by means of the diethylaminoethyl (DEAE)-dextran method (17) to verify expression of the recombinant protein. .. Cells were grown for two days in Dulbecco's modified Eagle medium (DMEM) (GIBCO BRL, Gaithersburg, MD, USA) supplemented with 7% fetal bovine serum (FBS) (GIBCO BRL) and antibiotics [100 units/mL benzylpenicillin (Wako, Osaka-city, Osaka, Japan), 100 μg/mL streptomycin sulfate (Wako), and 2.5 μg/mL fungison (GIBCO BRL)], and the transiently expressed proteins were detected immunologically as described in 2.3.

    Transfection:

    Article Title: Cyclic AMP (cAMP)-dependent proteolysis of GATA6 by proteasome: Zinc-finger domain of GATA6 has signals for nuclear export and proteolysis, both of which are responsive to cAMP.
    Article Snippet: .. Cell culture and transfection of expression plasmids Each plasmid construct was introduced into Cos-1 cells (ATCC, Manassas, VA, USA) by means of the diethylaminoethyl (DEAE)-dextran method (17) to verify expression of the recombinant protein. .. Cells were grown for two days in Dulbecco's modified Eagle medium (DMEM) (GIBCO BRL, Gaithersburg, MD, USA) supplemented with 7% fetal bovine serum (FBS) (GIBCO BRL) and antibiotics [100 units/mL benzylpenicillin (Wako, Osaka-city, Osaka, Japan), 100 μg/mL streptomycin sulfate (Wako), and 2.5 μg/mL fungison (GIBCO BRL)], and the transiently expressed proteins were detected immunologically as described in 2.3.

    Article Title: Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo
    Article Snippet: .. Plasmid transfection and expression analysis Expression levels of the plasmid construct were tested after transient transfection of RD cells (ATCC). .. Cells were plated in 60×15 mm tissue culture dishes at a density of 2.5×10 5 cells per well in complete Dulbecco’s modified Eagle’s medium plus 10% fetal bovine serum (HyClone, Thermo Scientific, Waltham, MA; GIBCO, Carlsbad, CA, USA) and allowed to adhere overnight.

    Article Title: Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo
    Article Snippet: .. Expression levels of the plasmid construct were tested after transient transfection of RD cells (ATCC). .. Cells were plated in 60×15 mm tissue culture dishes at a density of 2.5×10 5 cells per well in complete Dulbecco’s modified Eagle’s medium plus 10% fetal bovine serum (HyClone, Thermo Scientific, Waltham, MA; GIBCO, Carlsbad, CA, USA) and allowed to adhere overnight.

    Expressing:

    Article Title: Cyclic AMP (cAMP)-dependent proteolysis of GATA6 by proteasome: Zinc-finger domain of GATA6 has signals for nuclear export and proteolysis, both of which are responsive to cAMP.
    Article Snippet: .. Cell culture and transfection of expression plasmids Each plasmid construct was introduced into Cos-1 cells (ATCC, Manassas, VA, USA) by means of the diethylaminoethyl (DEAE)-dextran method (17) to verify expression of the recombinant protein. .. Cells were grown for two days in Dulbecco's modified Eagle medium (DMEM) (GIBCO BRL, Gaithersburg, MD, USA) supplemented with 7% fetal bovine serum (FBS) (GIBCO BRL) and antibiotics [100 units/mL benzylpenicillin (Wako, Osaka-city, Osaka, Japan), 100 μg/mL streptomycin sulfate (Wako), and 2.5 μg/mL fungison (GIBCO BRL)], and the transiently expressed proteins were detected immunologically as described in 2.3.

    Article Title: Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo
    Article Snippet: .. Plasmid transfection and expression analysis Expression levels of the plasmid construct were tested after transient transfection of RD cells (ATCC). .. Cells were plated in 60×15 mm tissue culture dishes at a density of 2.5×10 5 cells per well in complete Dulbecco’s modified Eagle’s medium plus 10% fetal bovine serum (HyClone, Thermo Scientific, Waltham, MA; GIBCO, Carlsbad, CA, USA) and allowed to adhere overnight.

    Article Title: Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo
    Article Snippet: .. Expression levels of the plasmid construct were tested after transient transfection of RD cells (ATCC). .. Cells were plated in 60×15 mm tissue culture dishes at a density of 2.5×10 5 cells per well in complete Dulbecco’s modified Eagle’s medium plus 10% fetal bovine serum (HyClone, Thermo Scientific, Waltham, MA; GIBCO, Carlsbad, CA, USA) and allowed to adhere overnight.

    Article Title: A Virus-Like Particle-Based Vaccine Candidate against the Tick-Borne Powassan Virus Induces Neutralizing Antibodies in a Mouse Model
    Article Snippet: POWV EDIII contains residues 301 to 401 of the EDIII protein and features a thrombin cleavage site and C-terminal octa-histidine tag (BEI Resources, Manassas, VA, USA) (Cat. NR-52391). .. A plasmid construct expressing POW-VLP was designed by ATCC and was synthesized by ATUM (Newark, CA, USA). ..

    Plasmid Preparation:

    Article Title: Cyclic AMP (cAMP)-dependent proteolysis of GATA6 by proteasome: Zinc-finger domain of GATA6 has signals for nuclear export and proteolysis, both of which are responsive to cAMP.
    Article Snippet: .. Cell culture and transfection of expression plasmids Each plasmid construct was introduced into Cos-1 cells (ATCC, Manassas, VA, USA) by means of the diethylaminoethyl (DEAE)-dextran method (17) to verify expression of the recombinant protein. .. Cells were grown for two days in Dulbecco's modified Eagle medium (DMEM) (GIBCO BRL, Gaithersburg, MD, USA) supplemented with 7% fetal bovine serum (FBS) (GIBCO BRL) and antibiotics [100 units/mL benzylpenicillin (Wako, Osaka-city, Osaka, Japan), 100 μg/mL streptomycin sulfate (Wako), and 2.5 μg/mL fungison (GIBCO BRL)], and the transiently expressed proteins were detected immunologically as described in 2.3.

    Article Title: Crystallization and X-ray diffraction analysis of SpaE, a basal pilus protein from the gut-adaptedLactobacillus rhamnosusGG
    Article Snippet: .. A plasmid construct containing residues 30–414 of the L. rhamnosus GG (ATCC 53103) spaE gene was generated in Escherichia coli BL21 (DE3) pLysS strain (Table 1; von Ossowski et al., 2010). ..

    Article Title: Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo
    Article Snippet: .. Plasmid transfection and expression analysis Expression levels of the plasmid construct were tested after transient transfection of RD cells (ATCC). .. Cells were plated in 60×15 mm tissue culture dishes at a density of 2.5×10 5 cells per well in complete Dulbecco’s modified Eagle’s medium plus 10% fetal bovine serum (HyClone, Thermo Scientific, Waltham, MA; GIBCO, Carlsbad, CA, USA) and allowed to adhere overnight.

    Article Title: Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo
    Article Snippet: .. Expression levels of the plasmid construct were tested after transient transfection of RD cells (ATCC). .. Cells were plated in 60×15 mm tissue culture dishes at a density of 2.5×10 5 cells per well in complete Dulbecco’s modified Eagle’s medium plus 10% fetal bovine serum (HyClone, Thermo Scientific, Waltham, MA; GIBCO, Carlsbad, CA, USA) and allowed to adhere overnight.

    Article Title: The Role of Treponema denticola Motility in Synergistic Biofilm Formation With Porphyromonas gingivalis
    Article Snippet: .. Each plasmid construct (10 μg) was linearized by digestion with NotI then electroporated into T. denticola ATCC 33520 cells as previously published (Li et al., ) with some modifications. .. Briefly, 2-days (exponential phase; A 650 of 0.16–0.28) T. denticola ATCC 33520 cultures were decanted into centrifuge tubes in the anaerobic chamber.

    Article Title: A Virus-Like Particle-Based Vaccine Candidate against the Tick-Borne Powassan Virus Induces Neutralizing Antibodies in a Mouse Model
    Article Snippet: POWV EDIII contains residues 301 to 401 of the EDIII protein and features a thrombin cleavage site and C-terminal octa-histidine tag (BEI Resources, Manassas, VA, USA) (Cat. NR-52391). .. A plasmid construct expressing POW-VLP was designed by ATCC and was synthesized by ATUM (Newark, CA, USA). ..

    Article Title: Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Staphylococcus aureus competence genes: Mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing1 Annette Fagerlund1, Per Einar Granum2, and Leiv Sigve Håvarstein1* 1Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, NO-1432 Ås, Norway.

    Article Title: Crystallization and X-ray diffraction analysis of SpaE, a basal pilus protein from the gut-adapted Lactobacillus rhamnosus GG
    Article Snippet: .. A plasmid construct containing residues 30–414 of the L. rhamnosus GG (ATCC 53103) spaE gene was generated in Escherichia coli BL21 (DE3) pLysS strain (Table 1 ; von Ossowski et al. , 2010 ). ..

    Construct:

    Article Title: Cyclic AMP (cAMP)-dependent proteolysis of GATA6 by proteasome: Zinc-finger domain of GATA6 has signals for nuclear export and proteolysis, both of which are responsive to cAMP.
    Article Snippet: .. Cell culture and transfection of expression plasmids Each plasmid construct was introduced into Cos-1 cells (ATCC, Manassas, VA, USA) by means of the diethylaminoethyl (DEAE)-dextran method (17) to verify expression of the recombinant protein. .. Cells were grown for two days in Dulbecco's modified Eagle medium (DMEM) (GIBCO BRL, Gaithersburg, MD, USA) supplemented with 7% fetal bovine serum (FBS) (GIBCO BRL) and antibiotics [100 units/mL benzylpenicillin (Wako, Osaka-city, Osaka, Japan), 100 μg/mL streptomycin sulfate (Wako), and 2.5 μg/mL fungison (GIBCO BRL)], and the transiently expressed proteins were detected immunologically as described in 2.3.

    Article Title: Crystallization and X-ray diffraction analysis of SpaE, a basal pilus protein from the gut-adaptedLactobacillus rhamnosusGG
    Article Snippet: .. A plasmid construct containing residues 30–414 of the L. rhamnosus GG (ATCC 53103) spaE gene was generated in Escherichia coli BL21 (DE3) pLysS strain (Table 1; von Ossowski et al., 2010). ..

    Article Title: Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo
    Article Snippet: .. Plasmid transfection and expression analysis Expression levels of the plasmid construct were tested after transient transfection of RD cells (ATCC). .. Cells were plated in 60×15 mm tissue culture dishes at a density of 2.5×10 5 cells per well in complete Dulbecco’s modified Eagle’s medium plus 10% fetal bovine serum (HyClone, Thermo Scientific, Waltham, MA; GIBCO, Carlsbad, CA, USA) and allowed to adhere overnight.

    Article Title: Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo
    Article Snippet: .. Expression levels of the plasmid construct were tested after transient transfection of RD cells (ATCC). .. Cells were plated in 60×15 mm tissue culture dishes at a density of 2.5×10 5 cells per well in complete Dulbecco’s modified Eagle’s medium plus 10% fetal bovine serum (HyClone, Thermo Scientific, Waltham, MA; GIBCO, Carlsbad, CA, USA) and allowed to adhere overnight.

    Article Title: The Role of Treponema denticola Motility in Synergistic Biofilm Formation With Porphyromonas gingivalis
    Article Snippet: .. Each plasmid construct (10 μg) was linearized by digestion with NotI then electroporated into T. denticola ATCC 33520 cells as previously published (Li et al., ) with some modifications. .. Briefly, 2-days (exponential phase; A 650 of 0.16–0.28) T. denticola ATCC 33520 cultures were decanted into centrifuge tubes in the anaerobic chamber.

    Article Title: A Virus-Like Particle-Based Vaccine Candidate against the Tick-Borne Powassan Virus Induces Neutralizing Antibodies in a Mouse Model
    Article Snippet: POWV EDIII contains residues 301 to 401 of the EDIII protein and features a thrombin cleavage site and C-terminal octa-histidine tag (BEI Resources, Manassas, VA, USA) (Cat. NR-52391). .. A plasmid construct expressing POW-VLP was designed by ATCC and was synthesized by ATUM (Newark, CA, USA). ..

    Article Title: Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Staphylococcus aureus competence genes: Mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing1 Annette Fagerlund1, Per Einar Granum2, and Leiv Sigve Håvarstein1* 1Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, NO-1432 Ås, Norway.

    Article Title: Crystallization and X-ray diffraction analysis of SpaE, a basal pilus protein from the gut-adapted Lactobacillus rhamnosus GG
    Article Snippet: .. A plasmid construct containing residues 30–414 of the L. rhamnosus GG (ATCC 53103) spaE gene was generated in Escherichia coli BL21 (DE3) pLysS strain (Table 1 ; von Ossowski et al. , 2010 ). ..

    Recombinant:

    Article Title: Cyclic AMP (cAMP)-dependent proteolysis of GATA6 by proteasome: Zinc-finger domain of GATA6 has signals for nuclear export and proteolysis, both of which are responsive to cAMP.
    Article Snippet: .. Cell culture and transfection of expression plasmids Each plasmid construct was introduced into Cos-1 cells (ATCC, Manassas, VA, USA) by means of the diethylaminoethyl (DEAE)-dextran method (17) to verify expression of the recombinant protein. .. Cells were grown for two days in Dulbecco's modified Eagle medium (DMEM) (GIBCO BRL, Gaithersburg, MD, USA) supplemented with 7% fetal bovine serum (FBS) (GIBCO BRL) and antibiotics [100 units/mL benzylpenicillin (Wako, Osaka-city, Osaka, Japan), 100 μg/mL streptomycin sulfate (Wako), and 2.5 μg/mL fungison (GIBCO BRL)], and the transiently expressed proteins were detected immunologically as described in 2.3.

    Generated:

    Article Title: Crystallization and X-ray diffraction analysis of SpaE, a basal pilus protein from the gut-adaptedLactobacillus rhamnosusGG
    Article Snippet: .. A plasmid construct containing residues 30–414 of the L. rhamnosus GG (ATCC 53103) spaE gene was generated in Escherichia coli BL21 (DE3) pLysS strain (Table 1; von Ossowski et al., 2010). ..

    Article Title: Crystallization and X-ray diffraction analysis of SpaE, a basal pilus protein from the gut-adapted Lactobacillus rhamnosus GG
    Article Snippet: .. A plasmid construct containing residues 30–414 of the L. rhamnosus GG (ATCC 53103) spaE gene was generated in Escherichia coli BL21 (DE3) pLysS strain (Table 1 ; von Ossowski et al. , 2010 ). ..

    Synthesized:

    Article Title: A Virus-Like Particle-Based Vaccine Candidate against the Tick-Borne Powassan Virus Induces Neutralizing Antibodies in a Mouse Model
    Article Snippet: POWV EDIII contains residues 301 to 401 of the EDIII protein and features a thrombin cleavage site and C-terminal octa-histidine tag (BEI Resources, Manassas, VA, USA) (Cat. NR-52391). .. A plasmid construct expressing POW-VLP was designed by ATCC and was synthesized by ATUM (Newark, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Staphylococcus aureus competence genes: Mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing1 Annette Fagerlund1, Per Einar Granum2, and Leiv Sigve Håvarstein1* 1Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, NO-1432 Ås, Norway.

    Amplification:

    Article Title: Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing.
    Article Snippet: This article is protected by copyright.. All rights reserved.. Staphylococcus aureus competence genes: Mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing1 Annette Fagerlund1, Per Einar Granum2, and Leiv Sigve Håvarstein1* 1Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, NO-1432 Ås, Norway.



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